By using the 20-200 μL micropipettor again, we extracted 100 μL from 6D and placed it into 6C. We then shook the plate gently so that the Tetrahymena will spread through its new media. Then, using the 20-200 μL micro pipettor, we transferred 100μM containing Tetrahymena from the middle compartment of the 24-well plate into column 6, row D. We filled rows A through D with the media. For this, we used the 100-1000 μL micro pipettor. We then transferred 900μM of the media in which Tetrahymena will later be placed from a container labeled PPT-9117 in column 6 of the 24 well plate. We first placed the 24 well plate on the dissecting microscope and focused on the middle compartment of the plate, which contained a sample of Tetrahymena. In today’s lab, we viewed a sample of Tetrahymena under both the dissecting and compound microscopes.
SERIAL DILUTION LAB CONCLUSION HOW TO
Another purpose of this lab was teaching us how to determine what micro pipettor we should use based on the amount of liquid we have, as well as to help us practice writing the methods and procedures for our experiment based on our hypothesis.
SERIAL DILUTION LAB CONCLUSION SERIAL
The purpose of this lab was to use dissecting microscopes to observe Tetrahymena and understand how serial dilution can be beneficial when it comes to accurately counting the number of cells in a certain solution, using dilution. We also focused on research and experimental design as well as designing our experiment. The objective of this lab was to use micropipettes and to conduct serial dilutions. Lab 5: Experimental design and Serial Dilution
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